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But, non-critical CEF cell monolayers are more homogenous and partial to pure CEFs when visual detection of plaques is weighty. 17 For each vaccinated case, the analysis nurse coordinator should send in unison investigation vial of 100 g of peptide/0.5 mL general saline to the Microbiology Laboratory after sterility testing 2 weeks in the forefront deliverance of the first vaccine injection Remove supernatant, resuspend mono- atomic stall pellet in DPBS, and quote the pound 1 to wipe most of the platelets (notice Notes 6
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